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Product IMX-TM3: Imagex-TGi Integrating Time-Gated CCD Imaging System

The Imagex-TGi Time-Gated CCD Imaging system is a complete integrated time-gated CCD camera system. It does not use a gated intensifier to implement time-resolution because the time-gating signals are applied directly to the CCD itself. This means that the resolution, noise performance and spectral sensitivity that you experience are those of a cooled CCD camera.

The Imagex-TGi system integrates all the frequency generation, light source triggering and delay functions into a single compact unit with a USB2.0 interface allowing complete control over all of the time-gating parameters and image readout.

 

The Imagex TGi is suitable for time-gated imaging of fluorophores of lifetimes of >c.100nanoseconds. For experiments with shorter lifetime materials we recommend the Imagex-nanoCCD that has enhanced nanosecond-scale control over the light source trigger delay. The Imagex-TGi system has been widely used for studying long-lifetime probes such as Lanthanide complexes, Oxygen and Temperature sensitive sensors and inorganic phosphors. Extiinction ratio of the Imagex TGi shutter is very high (c. 1:30,000 at 520nm) and is therefore very well-suited to eliminating short lifetime background signal to offer higher sensitivity detection of the longer-lifetime probe.

 

 

The figure on the right shows a particularly difficult optical arrangement tackled with an Imagex-TGi detection system. In this experiment we pipetted some samples of long-lifetime ruthenium nanospheres and of a shortlifetime rhodamine dye into a standard 96-well microplate.

We then illuminated the sample from below with an array of LEDs. This setup has the advantage in that the excitation light is delivered very efficiently to the sample. However it creates the problem that the light-source is pointing directly at the detector. This is something we would normally try to avoid!

 

 

The upper image shows the sample as it would be observed under normal ungated imaging conditions. The short lifetime rhodamine dye and the ruthenium nanospheres are broadly similar in emission intensity and colour. When we change over to gated imaging we effectively reject the short lifetime dye. We can increase the data collection time by a factor of 20 allowing the ruthenium samples to saturate the camera but still succesfully rejecting the ruthenium dye.

Imagex User Manual
imagex-TGi_and_nanoCCD_User_Manual.pdf
Adobe Acrobat document [3.3 MB]

Price:£11,000 (Currency=GBP)*

 

*Further 30% Discount Available for Educational, Government and Research Institutes. 

Price includes:Imagex System Unit, All cables, Universal Power Supply, Application Software, User Manual, Micromanager Device Apaptor, and Imagex Programmers Library with Example Source Code Application.

 

We can supply fixed price quotations with a validity of up to 12 months in the customer's own currency

Product IMX-NM3: Imagex-nanoCCD Integrating Time-Gated CCD Imaging System

 

The Imagex-nanoCCD Time-Gated CCD Imaging system has all the features of the Imagex-TGi but also provides two independently programmable 0-255 nanosecond delays on Channels 1 and 2  of its Lightsource Trigger outputs.

The ability to closely control the nanosecond delay on these trigger outputs opens up a range of new functionalities including nanosecond lifetime studies, pump-probe experiments and scattering/time-of-flight imaging.

In addition to the normal Imagex software application the Imagex-nanoCCD is also provided with a software library so that you can write your own Time-Gated Imaging application and integrate the system with other apparatus.

The Imagex nanoCCD has a fast switch-On response time (c.2nsec) Combining this fast response with a fast light source and the in-built programmable delay opens up a range of new experimental possibilities and allows the user to perform time-gating experiments with shorter lifetime fluorophores.

 

 

 

We have used the nanoCCD together with a nanosecond pulsed DPSS laser to perform lifetime imaging on a model series of quenched quinine sulphate solutions as shown in the accompanying figure.The above image shows a series of Quinine Sulphate solutions quenched with chloride ions. The solutions were pipetted into a black microwell plate and excited using an Elforlight actively Q-switched nanosecond pulsed laser (355nm)


The left-hand images ‘i)’ and ‘ii)’ show the ‘source’ intensity images measured with two different nanosecond delay settings on the laser trigger signal. The right-hand image shows a fluorescence lifetime image calculated from the two source images.

 

 

 

 

 

We compared the lifetimes calculated by the Imagex Software application with fitted data collected using A Time Correlated Single Photon Counting Spectrometer for the same set of samples. The data compare very favourably and suggest a linear response from the Imagex-nanoCCD system.

Imagex User Manual
imagex-TGi_and_nanoCCD_User_Manual.pdf
Adobe Acrobat document [3.3 MB]

Price:£11,750 (Currency=GBP)*

 

*Further 30% Discount Available for Educational, Government and Research Institutes. 

Price includes:Imagex System Unit, All cables, Universal Power Supply, Application Software, User Manual, Micromanager Device Apaptor, and Imagex Programmers Library with Example Source Code Application.

 

We can supply fixed price quotations with a validity of up to 12 months in the customer's own currency

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