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Imagex USB systems now out
Our Imagex range of Time-Gated CCD Imaging Systems now has an industry standard USB 2.0 interface.

Photonic Research Systems specializes in technology for measuring Long-Lifetime Fluorescent and Phosphorescent probes:

Some Examples of Long-Lifetime Probes include:

  • Lanthanide Chelates and Cryptates
  • Oxygen Sensors
  • Quantum Dots
  • Ethidium and Propidium Intercalating Agents
  • Inorganic Crystalline Phosphors
  • Upconverting Phosphors

 

At PRS we can offer a range of products and services to help you implement the ideal system for measuring your long-lifetime samples.

imagex launch panel

Time-Gated Imaging
The Imagex-TGi and Imagex-nanoCCD camera systems are directly-gated, cooled CCD imaging systems. They combine fast gating with low noise and high stability. Time-gating allows you to image long-lifetime labels even in samples where nothing can be seen with conventional imaging techniques.


All Imagex systems feature cooled CCD detectors, in-built frequency, delay and gate generators and frame readout circuitry. In the example below an Imagex-TGi system is driving a pulsed flashlamp at 300Hz to obtain high sensitivity images of an Europium cryptate-labelled DNA microarray. The nylon membrane used for DNA hybridisation in these images exhibits an intense, broad spectrum fluorescence when exposed to ultraviolet light. However because the nylon fluorescence is predominantly short lifetime (a few nanoseconds) it is sucessfully rejected by gated detection.

 

Time-Gated Imaging

  • High Selectivity
  • Excellent Background Rejection
  • Time-resolved Multiplexing
  • Improved Lifetime measurements

 

Fluorescence Lifetime Imaging (FLIM)
Imagex systems can also be used to measure Fluorescence Lifetime to generate new forms of contrast for singly and multiply-stained protein gels and blots. Because our systems do not use noisy intensifier technology our FLIM images have excellent signal-to-noise properties.

The Imagex nanoCCD features a programmable nanosecond delay unit. By varying the delay under software control the delay the user can collect a series of time-resolved images. Mathematical manipulation of these images allows Fluorescence Lifetime Images to be generated providing new forms of contrast and environmental sensitivity
.


The Imagex Script processor allows you to perform complex series of mathematical manipulations on your time-resolved images. Scripts allow the whole process of FLIM imaging to be automated. When you have your FLIM image the Imagex software can combine the Intensity image with the Lifetime image to produce an ‘Intensity/FLIM matrix image’...


These are some examples of nanosecond lifetime images collected using the Imagex nanoCCD. The light source used in both cases was a solid state diode pumped frequency doubled/tripled YAG laser with <1 nsec pulse width. The ability to display multiple images and create intensity/lifetime ‘matrix’ images is integrated into the  Imagex software package. PRS is currently assessing a number of nanosecond lightsources and is in the process of developing a turnkey FLIM system using this technology.


Fluorescence Lifetime Imaging

  • New Forms of Contrast
  • Environmental Sensitivity (O2,Ca2+,pH, Temperature)
  • Correct for quantum yield variations
  • Time-Resolved Multiplexing
  • FRET Imaging
  • Polarisation Imaging
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